Fig. 5.

Control: a topographic image of cytosolic side of OHC membrane patch treated with actin antibodies conjugated with secondary antibodies. b Corresponding phase image. c Height profile along the sectional line shown in image a. The distance between the two vertical dashed lines is 30.7 nm. The average value is 30.7 ± 4.0 nm obtained from different sections of the image b. d Particle size distribution. The solid line is a Gaussian fit to data. e Reconstructed tip shape. f A contour plot of the autocorrelation function of the background-subtracted gray scale image of a (see “Data acquisition and analysis” section)