Table 1.

Strains and plasmids used in this study

Strain or plasmid	Relevant characteristics*	Source or reference
P. gingivalis strains
ATCC 33277	Type strain, producing both FimA and Mfa1 fimbriae, Gmr	ATCC
SMF1	Derivative of ATCC 33277 with an insertional inactivation of the mfa1 gene, Emr	Lamont et al. (2002); Park et al. (2005)
JI-1	Derivative of ATCC 33277 with fimA deletion by cat, Cmr	This study
JI-2	Derivative of ATCC 33277 with an insertional inactivation of the mfa2 gene, Emr	This study
JI-3	JI-2 containing pTCBex-mfa2, a complemented strain, Emr, Tcr	This study
JI-12	Derivative of JI-2 with fimA deletion by cat, Emr, Cmr	This study
JI-4	JI-12 containing pTCBex-mfa2, a complemented strain, Emr, Cmr, Tcr	This study
E. coli strains
DH5 α	F−φ80lacZ ΔM15 Δ(lacZYA-argF) U169 deoR recA1endA1hsdR17(,) phoA supE44 l−thi-1gyrA96relA1	Takara
BL21	F−ompT hsdSB (,) gal dcm araB : : T7 RNAP-tetA	GE Healthcare Bio-Science
S17-1	RecA−, ΩRP4-Tc : : Mu-Kn : : T7), Tpr	Gardner et al. (1996)
TOP10	F−mcrA Δ(mrr-hsdRMS-mcrBC) F80lacZ ΔM15 ΔlacC74 recA1 araD139 Δ(ara-leu) 7697 galU galK rpsL (Strr) endA1 nupG	Invitrogen
Plasmids
pCR-TOPO	Cloning vector, Kmr	Invitrogen
pCRJI-2	pCR-TOPO derivative carrying a 1.6 kb fragment containing mfa2	This study
pVA2198	Plasmid used for a drug cassette, ermF-ermAM, Emr	Fletcher et al. (1997)
pTCBex	Derivative of pT-COW, an expression vector, containing the promoter region of fimR, Apr in E. coli, Tcr in P. gingivalis	Nagano et al. (2007)
pTCBex-mfa2	pTCBex derivative carrying the coding region of mfa2, Apr in E. coli, Tcr in P. gingivalis	This study
pGEX-6P	GST gene fusion and expression vector, Apr	GE Healthcare Bio-Science

*ATCC, American Type Culture Collection; Gm^r, gentamicin resistance; cat, chloramphenicol acetyltransferase; Cm^r, chloramphenicol resistance; Em^r, erythromycin resistance; Tc^r, tetracycline resistance; Tp^r, trimethoprim resistance; Km^r, kanamycin resistance; Ap^r, ampicillin resistance.