Table 3.
Identification of proteins by MALDI-TOF MS peptide mass fingerprinting
Protein number* | Probability† | Description (corresponding CDS‡ number in P. gingivalis ATCC 33277 genome database) (Naito et al., 2008) | Mr (kDa) | Accession number§ |
---|---|---|---|---|
1 | 111 | von Willebrand factor A domain protein (pgn0291) | 134.65 | 34540039 |
2 | 90 | |||
3 | 71 | Pg-II fimbriae a (pgn0287)|| | 60.79 | 22255314 |
4 | 51 | |||
5 | 101 | |||
6 | 53 | |||
7 | 93 | |||
8 | 77 | |||
9 | 88 | Lipoprotein, putative (pgn0289) | 50.01 | 34540037 |
10 | 81 | Immunoreactive 32 kDa antigen PG49 (pgn0290) | 37.14 | 34540038 |
*Numbers refer to designations of protein bands in Fig. 3.
†Probabilities were based on the molecular weight search (MOWSE) scoring algorithm.
‡Protein-coding sequence.
§Nonredundant protein database at the NCBI (http://www.ncbi.nlm.nih.gov/).
||Pg-II fimbriae a is the gene product of Pg-II fim a (designation from Ogawa et al., 1995) from P. gingivalis strain BH18/10 and is another name for the Mfa1 protein, both of which were originally called minor fimbriae (Hamada et al., 1996).