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. Author manuscript; available in PMC: 2011 Jan 14.
Published in final edited form as: J Med Chem. 2010 Jan 14;53(1):325–334. doi: 10.1021/jm901268n

Figure 5.

Figure 5

Effects of 10i on mitotic spindles and cell cycle distribution. HeLa cells were treated with vehicle (A) or 0.50 µM 10i (B) for 18 h, fixed and cellular structures visualized by indirect immunofluorescence. Cellular microtubules were visualized with a β-tubulin antibody (green), centrosomes using a γ-tubulin antibody (red), and DNA using DAPI (blue). For the cell cycle distribution studies, MDA-MB-435 cells were treated for 18 h with vehicle (A) or 0.50 µM 10i then stained with Kirshan’s reagent and evaluation by flow cytometry.