Table 1.

Primer sequences and PCR conditions.

	Primer sequences	Denat. t/T	Anneal. t/T	Extens. t/T	No. of cycles
β-actin	sense 5′-ATC TGG CAC CAC ACC TTC TAC AAT GAG CTG CG–3′	1 min/94°C	1 min/59°C	1.5 min/72°C	25
	antisense 5′-CGT CAT ACT CCT GCT TGC TGA TCC ACA TCT GC–3′
IL-6	sense 5′-ATG AAC TCC TTC TCC ACA AGC GC-3′	1 min/94°C	1 min/51°C	1.5 min/72°C	30
	antisense 5′-GAA GAG CCC TCA GGC TGG ACT G-3′
TF	sense 5′-ACT ACT GTT TCA GTG TTC AAG CAG TGA TTC-3′	1 min/94°C	1 min/52°C	1.5 min/72°C	35
	antisense 5′-ATT CAG TGG GGA GTT CTC CTT CCC AGC TCTG-3′

t/T: time and temperature, Denat.: denaturation, Anneal.: annealing, Extens.: extension.