TGFβ2-induced expression of α-SMA and calponin. (A) Clones 8 and 5, the parental HPVEC and HDMECs were grown for 10 days in absence (control, lane 1) or presence of 2ng/ml of TGFβ1 (lane 2), TGFβ2 (lane 3) or TGFβ3 (lane 4). Cell lysates were analyzed by western blot for the expression of CD31, α-SMA and calponin. (B) Clone 8 (left panels) and Clone 5 (right panels) were grown for 10 days in absence (control; top panels) or presence of 2ng/ml of TGFβ2 (TGFβ2; bottom panels). Cells were double-labeled with goat anti-human CD31/FITC-conjugated secondary Ab and mouse anti-α-SMA/Texas Red-conjugated secondary Ab. α-SMA positive cells, which also expressed CD31, were counted to determine number of positive cells in each clone. * P values<0.05.