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. Author manuscript; available in PMC: 2010 Jan 24.
Published in final edited form as: Nat Struct Mol Biol. 2008 Apr 13;15(5):469–476. doi: 10.1038/nsmb.1403

Figure 6.

Figure 6

The HSA, post-HSA and protrusion 1 domains are essential for viability. TRP1-marked plasmids bearing wild-type STH1, sth1Δ301–383, sth1Δ385–392, sth1Δ651–697 or sth1Δ301–359 (p976, p1724, p1683, p2500 or p2465, respectively) were transformed into an sth1Δ strain (YBC943) covered by wild-type STH1 on a URA3-marked plasmid (p114). Loss of the URA3-marked plasmid is enforced on medium containing 5′ fluoroorotic acid (FOA), which prevents the growth of Ura3+ cells. Inability to lose the URA3-marked plasmid bearing wild-type STH1 demonstrates the lack of complementation by the sth1 domain deletion derivative. Vector, empty vector control.