Figure 1.
Fluorescence responses of 5 µM MitoPY1 to various reactive oxygen species (ROS). Bars represent relative responses at 0, 5, 15, 30, 45, and 60 min after addition of each ROS. Data shown are for 10 mM O2−, 200 µM NO, and 100 µM for all other ROS. Data were acquired at 25 °C in 20 mM HEPES, pH 7, with excitation λ = 503 nm and emission collected between 510 and 750 nm.