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. Author manuscript; available in PMC: 2010 Jan 24.
Published in final edited form as: J Am Chem Soc. 2008 Jul 8;130(30):9638–9639. doi: 10.1021/ja802355u

Figure 2.

Figure 2

Confocal fluorescence images of live HeLa cells with increases in mitochondrial H2O2 levels visualized using MitoPY1. Images displayed represent emission intensities collected in optical windows between 527–601 nm upon excitation at 510 nm for MitoPY1. HeLa cells incubated with 5 µM MitoPY1 for 60 min at 37 °C and imaged with MitoPY1 (a), MitoTracker Red and Hoechst (overlay, b), and MitoPY1 with MitoTracker Red (overlay, c). HeLa cells incubated with 5 µM MitoPY1 for 60 min at 37 °C with 100 µM H2O2 added for the final 40 min and imaged with MitoPY1 (d), MitoTracker Red and Hoechst (overlay, e), MitoPY1 and MitoTracker Red (overlay, f), and brightfield (g) with 20 µm scale bar. HeLa cells incubated with 5 µM MitoPY1 for 60 min at 37 °C and imaged with MitoPY1 (h), MitoTracker Red and Hoechst (overlay, i), and MitoPY1 with MitoTracker Red (overlay, j). HeLa cells incubated for 24 h with 1 mM paraquat, then washed and incubated with 5 µM MitoPY1 for 60 min at 37 °C and imaged with MitoPY1 (k), MitoTracker Red and Hoechst (overlay, l), MitoPY1 and MitoTracker Red (overlay, m), and brightfield (n) with 20 µm scale bar.