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. Author manuscript; available in PMC: 2010 May 1.
Published in final edited form as: J Immunol. 2009 Nov 1;183(9):5458–5467. doi: 10.4049/jimmunol.0803217

Figure 4. Expression of FoxP3 is not stably maintained by 1,25(OH)2D3.

Figure 4

(A) Purified CD4+CD25− T cells were stimulated with anti-CD3/CD28 coated beads in the presence of 100nM 1,25(OH)2D3 or vehicle control for the times shown. Cells were analysed for expression of CTLA-4 and FoxP3. (B) Expression was determined for both the total and re-cycling pool of CTLA-4. Dotted lines indicate expression in the presence of 1,25(OH)2D3 whereas black lines indicate vehicle control. The shaded histogram represents isotype control staining. (C) CFSE labelled CD4+ CD25− T cells were stimulated as above and analysed for CTLA-4 expression in combination with cell division. Numbers in quadrants refer to percentage of cells. Data shown is from a single experiment representative of 3 carried out.