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. Author manuscript; available in PMC: 2010 May 1.
Published in final edited form as: J Immunol. 2009 Nov 1;183(9):5458–5467. doi: 10.4049/jimmunol.0803217

Figure 6. T cell culture in the presence of 1,25(OH)2D3 and IL-2, results in suppressive function.

Figure 6

Suppressor CD4+ T cells were generated by stimulating purified CD4+CD25− with autologous monocytes plus anti-CD3 in the presence of 100nM 1,25(OH)2D3 and addition of IL-2 (200IU/ml) for 7 days. Cells cultured without 1,25(OH)2D3 and IL-2 were used as mock-suppressor populations. Suppressor mock-suppressor populations were added at a ratio of 1:10 CFSE labelled responder T cells and stimulated by dendritic cells plus anti-CD3 at a ratio of 1:5 DCs : T cells. Responses were also compared to unstimluated, (resting CD4+ cells) as suppressor controls. Responder CFSE profiles are shown with the % cells entering cell division (left panels) along with the phenotype of suppressor and mock-suppressor cells (right panel).CFSE profiles are from a single experiment of four performed. Data showing individual experiments are summarised as median fluorescence intensity (CFSE responder MFI) in the top right panel. Data were analysed using the Mann-Whitney test. Dot plots show typical expression of CTLA-4 and FoxP3 in cells with and without treatment with 1,25(OH)2D3 plus IL-2.