FIGURE 3.

Invariable E-selectin ligand expression on NK cells in CHS. CHS responses using sensitization and challenge applications of DNFB were generated in wt, PSGL-1^−/−, FT4/7^−/−, or Rag1^−/− mice. ILN draining DNFB- or vehicle-treated skin from wt and PSGL-1^−/− mice or spleens from DNFB- or vehicle-treated FT4/7^−/− and Rag1^−/− mice were harvested, and ESL expression was assayed on DX5⁺ NK1.1⁺ NK cells by flow cytometry. A, In representative scatter plots, a comparable percentage of ESL⁺ DX5⁺ NK1.1⁺ NK cells was observed from both untreated and DNFB-treated mice. These percentages (mean ± SD) were consistently ~50% (B). Comparing the absolute number of ESL⁺ NK cells with ESL⁺ T cells from untreated or DNFB-treated mice showed that ESL⁺ T cells were 10-fold greater than ESL⁺ NK cells in DNFB-treated mice (C). In PSGL-1^−/− mice, there was a 50% lower level of E-selectin-Ig staining on NK cells, while NK cells deficient in FT4/7 did not express any E-selectin ligand activity (D). Rag1^−/− mice expressed a similar percentage of ESL⁺ DX5⁺ NK1.1⁺ NK cells as wt mice (D). *, Percent of DX5⁺ NK1.1⁺ gated cells in Rag1^−/− mice was 10-fold higher than in wt controls. Experiments were performed a minimum of three times.