(A) Schematic diagram of the in vitro trans-endothelial migration assay. Conditioned media were placed in the bottom well. Test cells were plated in the top trans-well chamber. A confluent monolayer of human endothelial cells was present as indicated. Quantification was performed as described in the experimental procedures.
(B) Trans-endothelial migration of LM2 and A375-BoM2 cells in the presence of media conditioned by the indicated cell lines. n= 60–150.
(C) Trans-migration activity of tumor cells under indicated conditions was expressed as a fold difference relative to migration of parental MDA231 or A375 cells in the presence of media from non-tumorigenic cells (MCF10A conditioned media for MDA231; HaCat conditioned media for A375). n= 40–70.
Error bars in all cases represent SEM and p values were based on two-tailed Mann-Whitney test.