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. Author manuscript; available in PMC: 2010 Dec 24.
Published in final edited form as: Cell. 2009 Dec 24;139(7):1315–1326. doi: 10.1016/j.cell.2009.11.025

Figure 4. Tumor-derived mediators of cancer cell attraction.

Figure 4

(A) Schematic diagram of the in vitro trans-endothelial migration assay. Conditioned media were placed in the bottom well. Test cells were plated in the top trans-well chamber. A confluent monolayer of human endothelial cells was present as indicated. Quantification was performed as described in the experimental procedures.

(B) Trans-endothelial migration of LM2 and A375-BoM2 cells in the presence of media conditioned by the indicated cell lines. n= 60–150.

(C) Trans-migration activity of tumor cells under indicated conditions was expressed as a fold difference relative to migration of parental MDA231 or A375 cells in the presence of media from non-tumorigenic cells (MCF10A conditioned media for MDA231; HaCat conditioned media for A375). n= 40–70.

Error bars in all cases represent SEM and p values were based on two-tailed Mann-Whitney test.