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. Author manuscript; available in PMC: 2010 Jul 31.
Published in final edited form as: J Immunol Methods. 2009 May 14;346(1-2):38–44. doi: 10.1016/j.jim.2009.05.003

Fig. 3.

Fig. 3

CD1d-based aAPC can be used to expand human NKT cells. T cells (CD3+)were isolated from PBMC using magnetic bead separation. The cells were stimulated weekly with irradiated PBMC pulsed with 100ng/ ml α-GalCer (10:1 ratio) or a-GalCer loaded aAPC± anti CD28 (1:1 ratio). Data shown are after 2 weeks of stimulation.