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. Author manuscript; available in PMC: 2010 Dec 7.
Published in final edited form as: Circ Res. 2009 Oct 22;105(12):1248–1255. doi: 10.1161/CIRCRESAHA.109.208926

Figure 5. Blockade of PKC and PI3-kinase on Ang II-induced P47phox phosphorylation in SHR and WKY rat neurons.

Figure 5

Phosphorylation of p47phox was measured by using immunoprecipitation with Anti-p47phox antibody followed by immunoblotting with anti-phosphoserine antibody in SHR and WKY rat neurons treated with the following conditions: control, Ang II (Ang, 100 nmol/L), Ang II plus GF109203 (Ang+GF, 1 μmol/L), and Ang II plus LY294002 (Ang+LY, 10 μmol/L) or Ang II plus combination of GF10923 with LY294002 (Ang+GF+LY). Bottom: representative blot showing the levels of phosphorylated p47phox and total p47phox; Top: bar graphs showing the mean level of phosphorylated p47phox in SHR and WKY neurons under each treatment condition. Data are mean±SE (n=5 experiments). * p <0.01 versus respective control. # p <0.05 versus Ang II treatment.