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. Author manuscript; available in PMC: 2010 May 15.
Published in final edited form as: Dev Biol. 2009 Mar 3;329(2):242–257. doi: 10.1016/j.ydbio.2009.02.024

Figure 12. Hypertrophic chondrocytes expressing P-SMAD1/5/8 are increased in nm GP compared to wt.

Figure 12

A. Immunocytochemical staining with P-SMAD 2 and P-SMAD1/5/8 specific antibodies showed comparable levels of P-SMAD2 and increased levels of P-SMAD1/5/8 in hypertrophic chondrocytes of E9 nm GP with respect to wt. Sections were counterstained with DAPI. B. The numbers of P-SMAD2- and P-SMAD1/5/8- positive cells relative to total numbers of cells (DAPI-positive nuclei) in seven independent fields each from wt and nm limbs were determined using ImageJ software. Data was analyzed for statistical significance using the Student's t-test.