Skip to main content
. Author manuscript; available in PMC: 2010 May 15.
Published in final edited form as: Dev Biol. 2009 Mar 3;329(2):242–257. doi: 10.1016/j.ydbio.2009.02.024

Figure 2. Expression of matrix protein genes.

Figure 2

A. Expression of collagen type IX (COL9A1) in E12 tibia sections from wt and nm chicks detected by in situ hybridization. Three sections from each of 10 embryos were analyzed with consistent results. B. Higher magnification of E12 limb sections hybridized with probes for collagen type IX (COL9A1), aggrecan (AGC1), collagen type X (COL10A1), and collagen type II (COL 2A1). In situ hybridizations with DIG-labeled probes were performed on 40 μm tibia sections. When necessary, cartilage element limits were outlined. P: proliferative zone; PH pre-hypertrophic zone; H: hypertrophic zone. Scale bar: 1mm.