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Quantitation of TNFR1 and TNFR2 mRNA expressions in human fetal astrocytes. Total RNA was isolated from astrocytes and THP-1 cells using a Trizol reagent. TNFR1 and TNFR2 mRNA levels were analyzed by competitive RT-PCR and calculated using densitometry. Different copies (10-104) of competitors were used in competitive RT-PCR. Experiments were performed using at least three different batches of human fetal brain samples.
