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TNFR1 blocking antibody inhibits TNF-α dependent NF-κB activation in human astrocytes. Human astrocytes were preincubated for 1 h with TNFR1 (5 µg/mL or 0.5 µg/mL) or TNFR2 blocking antibodies (5 µg/mL), and then treated with TNF-α (100 U/mL) for 30 min at 37℃. The cells were then examined for NF-κB activation by using an electrophoretic mobility shift assay.
