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MG1655dnaC2 was synchronized by incubation at the nonpermissive temperature. At 0 min, the culture was divided into two equal parts. Either no addition (A) or cCM was added to a final concentration of 2× after the cultures were shifted to the permissive temperature (30°C) for 10 min and returned to the nonpermissive temperature (38°C) for a further 5-min incubation (B). Samples were taken for flow cytometry at the times indicated.
