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SDS gel electrophoretic analyses of purified recombinant human one-chain and two-chain carboxylases. Affinity-purified one-chain carboxylase (lanes 1 and 5), two-chain carboxylase (lanes 2 and 6), and NEM-labeled two-chain carboxylase (lanes 3 and 7) were fractionated by SDS–PAGE under nonreduced (NR) and reduced (R) conditions and visualized by silver stain. The N-terminal and C-terminal peptides are indicated by arrows.
