Figure 6.

Re-evaluation of the immunomodulatory properties of MAb 4-10A. (A) BIAcore SPR analysis of S. mutans adherence to salivary agglutinin in the presence of sera collected from BALB/c mice immunized with S. mutans alone or with ICs containing MAb 4-10A at the indicated dilutions. For the sake of clarity the change in resonance units detected over the 60 second injection cycle rather than the complete sensograms are shown. (B) MAb 1-6F competition ELISA. Sera collected from mice 7 days after primary immunization with S. mutans alone or with ICs containing MAb 4-10A at the indicated dilutions were tested for their ability to inhibit binding of biotin-labeled 1-6F to S. mutans whole cells. Results are expressed as the mean percent inhibition ± SEM and statistical significance compared to serum from S. mutans only immunized mice is indicated by P values. (C) Quantitative anti-NR21 IgG subclass ELISA. The levels of NR21-specific IgG1, IgG2a, and IgG2b subclass antibodies were measured in the same sera described in panel B. Results are expressed as mean ± SEM and statistical significance compared to serum from S. mutans only immunized mice is indicated by P values. (D) MAb 4-10A enhancement of MAb 1-6F binding to S. mutans whole cells by ELISA. The percent increase or decrease in binding of biotin-labeled MAb 1-6F to S. mutans in the presence of MAb 4-10A added at the indicated dilution was calculated. Data are representative of three independent experiments. Statistical analysis was performed using graph pad prism 4.0 and analysis included one-way anova.