Table 1.
data set namea | replicates | average no. of MS/MS scans | database search tools | precursor/fragment m/z tolerance | databases used for searchb |
---|---|---|---|---|---|
Score Combination Evaluation Data Sets | |||||
Serum Orbi | 5 | 6548 | MyriMatch, Sequest, Mascot | 0.1/0.5 | ipi.HUMAN.v3.47 |
Plasma QSTAR | 5 | 7058 | MyriMatch, Sequest, Mascot | 0.25/0.25 | ipi.HUMAN.v3.47 |
DLD1 LTQ | 4 | 12913 | MyriMatch, Sequest, Mascot | 1.25/0.5 | ipi.HUMAN.v3.47 |
Peptide Segregation Evaluation Data Sets | |||||
DLD1 LTQ | 4 | 12913 | MyriMatch | 1.25/0.5 | ipi.HUMAN.v3.47 |
Serum Orbi | 5 | 6548 | MyriMatch | 0.1/0.5 | ipi.HUMAN.v3.47 |
Parsimony Performance Evaluation Data Sets | |||||
DLD1 LTQ | 4 | 12913 | MyriMatch | 1.25/0.5 | uniprot_sprot-rel56.2 |
Serum Orbi | 5 | 6548 | MyriMatch | 0.1/0.5 | uniprot_sprot-rel56.2 |
Data set names represent sample type and the mass spectrometer used in the analysis (see Materials and Methods for additional details).
All protein databases contained reversed sequence entries (decoys) for estimation of false discovery rates. Exhaustive database search configurations were made available in Supplemental File 2.