Figure 1.

Schematic representation of constructs employed in this study. HIgR, PRR1, and hPVRα represent the full-length molecules encoded by cDNAs. V-TM(PRR1) is an internally deleted version of PRR1, where the two C domains were deleted. V(HIgR)-PVRα is a chimeric protein formed of the V domain of HIgR fused to the CC domains and transmembrane and cytoplasmic region of hPVRα. sVCC(HIgR)-Fc, sV(HIgR)-Fc, sVCC(PVRα)-Fc, and CTLA4-Fc represent soluble forms of receptors constructed by fusion of the VCC or V domains of HIgR, or the V domain of CTLA4, with the Fc portion of human IgG1. Construction is described in Materials and Methods.