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(A) Binding of mAb R1.302 to the soluble form of HIgR—sVCC(HIgR)-Fc—containing the entire ectodomain (VCC) fused to Fc. (B) Mapping of the epitope recognized by the mAb R1.302 to the V domain of HIgR. sVCC(HIgR)-Fc (•), sV(HIgR)-Fc (▴), or sVCC(PVRα)-Fc (■), immobilized to microwells through anti-human IgGs, were reacted with serial dilutions of biotinylated mAb R1.302. Abscissa represents the mAb dilutions (mAb Dil.) expressed as log10.
