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. Author manuscript; available in PMC: 2010 Mar 1.
Published in final edited form as: Free Radic Biol Med. 2008 Dec 24;46(5):672–679. doi: 10.1016/j.freeradbiomed.2008.12.006

FIGURE 6.

FIGURE 6

Expression assay of some selected genes in 661W cells pre-treated with curcumin followed by exposing to H2O2. Pre-treatment with curcumin was for 3h followed by thorough washing off the media and after 3h of washing the cells were exposed to 0.5 mM of H2O2. Cells were harvested after 3h of H2O2 treatment and RNA isolated for quantitative RT-PCR. The expression data was analyzed by comparative Ct value method after normalizing against house keeping gene, Hprt. Expression values (±SD) are presented against no-treatment value which is 100% (n = 4 sample × 3 replication assay per sample). *P < 0.001, by Student’s t-test.