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. Author manuscript; available in PMC: 2010 Jan 25.
Published in final edited form as: J Biol Chem. 2004 Jun 20;279(34):35377–35383. doi: 10.1074/jbc.M400354200

FIG. 3. Two-dimensional gel electrophoresis of the PATRR plasmid.

FIG. 3

A, psPATRR11 prepared by the alkaline lysis method. B, psPATRR11 treated with topoisomerase I in the presence of various amounts of ethidium bromide. C, topoisomerase I-treated psPATRR11 digested with T7 endonuclease. D, topoisomerase I-treated psPATRR11 digested with S1 nuclease. Two downward-sloping curves originating from cruciform extrusion are observed at the lower right side on both the A and the B gels, but neither are observed in the C nor in the D gel. Spots at the upper left side on the gels originate from open circular nicked plasmids, whereas spots near the center of the gels are from nuclease-cleaved linear plasmids.