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. Author manuscript; available in PMC: 2010 Jan 25.
Published in final edited form as: J Biol Chem. 2004 Jun 20;279(34):35377–35383. doi: 10.1074/jbc.M400354200

FIG. 4. Mapping of nuclease cleavage sites in the PATRR.

FIG. 4

A, mapping by digestion with restriction enzyme. S1, S1 nuclease; T7, T7 endonuclease; RE, restriction enzyme. B, restriction map of the PATRR-flanking region. C, mapping by sequencing. The entire PATRR is shown as a putative hairpin structure predicted by mfold software (mfold.burnet.edu.au/dna_form). The arrows indicate cleavage sites with T7 endonuclease, whereas the arrowheads indicate those with S1 nuclease. the relatively GC-rich region is indicated by a bracket.