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. Author manuscript; available in PMC: 2010 Jan 25.
Published in final edited form as: J Biol Chem. 2004 Jun 20;279(34):35377–35383. doi: 10.1074/jbc.M400354200

FIG. 6. Gel electrophoresis mobility shift assay of the PATRR fragment.

FIG. 6

psPATRR11 (lanes 1–3) or pΔPATRR11 (lanes 4–6) prepared by the Triton lysis method was incubated at room temperature for 7 days and then reacted with anti-cruciform antibody, 2D3. For psPATRR11, another monoclonal antibody was used as a control (lanes 7–8). psPATRR11 without incubation at room temperature was also reacted with 2D3 (lanes 9–10). The concentration of 2D3 antibody is indicated at the bottom. The arrow indicates the shifted band.