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A, an AFM image of psPATRR11 prepared by the Triton lysis method. B, an AFM image of the psPATRR11 after incubation at room temperature. C, another image of psPATRR11 at a higher magnification. D, an AFM image of psPATRR11 after DNA cross-linking followed by restriction digestion. The arrows indicate the cruciform extrusion. Longer fragments without cruciform structure may originate from the cloning vector. The scale bar is indicated in μm.
