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. 2009 Nov 12;38(2):414–427. doi: 10.1093/nar/gkp1027

Figure 2.

Figure 2.

Activity of a p66 promoter-signal sequence-phoA fusion reporter in the presence of candidate regulators. phoA E. coli carrying the p66 promoter-signal sequence-phoA fusion, were transformed with plasmids encoding nine candidate B. burgdorferi regulatory proteins or the vector only control, and were tested for AP activity. Shown are the means ± standard deviations of three to five replicate experiments. To incorporate all data from independent experiments, activities in the presence of the candidate regulators were normalized to that of the vector only control. Statistically significant differences were observed for BBA23 (light bar, increased activity), and for BB0232, and BB0527 (black bars, decreased activity) with a P-value of less than 0.01 based on a Students’ two-tailed t-test.