Figure 3.

Interaction of purified Hbb, BBA23 and BB0527 with the p66 promoter region. Various concentrations of purified recombinant proteins were incubated with 100 pM radiolabeled DNA for 30 min, and the reactions were run on nondenaturing 5% polyacrylamide gels. The gels were dried and exposed to film overnight at −70°C and were visualized by autoradiography. Hbb starts to shift the p66 promoter fragment at 1 nM (arrow), while BB0527 requires much higher concentrations, and BBA23 did not appear to interact with this DNA fragment. The p66 promoter fragment is homogeneous when analyzed by agarose gel electrophoresis but generally heterogeneous by acrylamide gel electrophoresis, even after gel purification.