Figure 4.

Specificity of interaction of purified Hbb with B. burgdorferi DNA fragments. (A) Varying concentrations of Hbb were incubated with 100 pM radiolabeled DNA fragments, and the samples fractionated on non-denaturing gels, as described for Figure 3. Arrows indicate the first shifted band apparent for each DNA fragment, all of which appear at 1–2 nM Hbb. (B) One microgram salmon sperm DNA was added to each binding reaction as a competitor. (C) Hbb was held constant, and unlabeled DNA fragments were added at the concentrations shown.