. 2009 Nov 12;38(2):414–427. doi: 10.1093/nar/gkp1027

Table 3.

Oligonucleotides used for cloning and sequencing

Target, purpose	Genome coordinates	Forward primer, 5′–3′	Reverse primer, 5′–3′
bba23 cl,seq^a	15303–15886	tagcggatccaaaatgtttattgagaaaatattacaaagc	tacgctcgagtcatggttgggtttgggc
bb0225 cl,seq^a	229242–230266	tagcggatccatagccccaatggtaaacattacagacg	tacgctcgagtcattcctaaaagtttggttaagtatacaggc
bb0232 cl,seq^a^,^b	237956–237620	tagcggatccatgtctttttcaagaagaccaaagg	tacgctcgagtcataaccggcatttaacctttgatacc
bb0345 cl,seq^a	354572–353303	tagcggatccttgactcttgaaatggtagctgagg	tacgctcgagtcagcaattataactcttgttttaccacttcc
bb0355 cl,seq^a	365570–365181	tagcggatcctatccaatgcatggagtaggtacg	tacgctcgagtcaatcttaaagaatcaaacacagcaaatcc
bb0462 cl,seq^a^,^b	484254–484820	tagcggatccttggagcaagtgaagttctggagg	tacgctcgagtcaacctctcctaactccatctgg
bb0468 cl,seq^a	488226–489096	tagcggatccttgaatgtaagagatttgtcttttaagc	tacgctcgagtcatgaacttaatttaacatactttgcaacc
bb0527 cl,seq^a	537538–538491	tagcggatcctcagaattgataattgatattggaaataccagc	atcgctcgagtcacgtagcaaggaaaattatgtagaatcaaacg
bb0647 cl,seq^a^,^b	686504–685854	tagcggatccgacaacataatagacgtacattcc	tacgctcgagtcatgtcaatttcttctatgtttttagg
His6-HA-HA T^c cloning	n/a	tatgcaccaccaccaccaccacaccggttatccttacgacgtacctg actacgcagcaggatacccatacgacgtcccagactacgctggtac
His6-HA-HA B^c cloning	n/a	cagcgtagtctgggacgtcgtatgggtatcctgctgcgtagtca ggtacgtcgtaaggataaccggtgtggtggtggtggtggtgca
bb0225seq^d	229722,229294	ttattgtacatgcaagg	ttgtaatcactagataaaggcc
bb0345seq^d	353840,354168,353867	aaattgccgagcagc	1 atctccctctttaagagaaacg 2 ttcccttaacatcaaactgcc
bb0468seq^d	488607, 488724	atggcggagttaatctagg	attgcaaccttattcaccg
bb0527seq^d	538038,538042	1 agaattgataattgatattggaaatacc 2 attccccattagcactcc	ttggagtgctaatggg
p66 promoter cloning	626808–627175	agaggatcctggacctttacaaacacaaatggca	agaggatcctttaatgcgtctgctgcaaata

Restriction sites are underlined. n/a: not applicable.

^acl,seq denotes that the oligonucleotides were used for both cloning and sequencing.

^bThese primers were described in a previous study (44) but are included here for the sake of convenience.

^cThese oligonucleotides were annealed to form the His6-HA-HA tag inserted in the cloning vector pET30a, and do not correspond to sequences in the B. burgdorferi genome.

^dThese oligonucleotides were used for internal sequencing of candidate clones.