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Binding of UvrA to the duplex containing the site-specific 5R-Tg lesion paired with dA. In (A) and (B), UvrA at the indicated concentrations was incubated with 2-nM substrates at 37°C for 20 min in UvrABC binding buffer and then analyzed on a 3.5% polyacrylamide native gel by gel mobility shift assays. (A) TG-51 bp; (B) AAF-50 bp. (C) The binding curves generated based on the titration data in (A) and (B).
