Figure 2.

Incisions of the site-specific 5R-Tg paired with dA substrate by UvrABC nuclease. In (A) and (B), 2-nM DNA substrates 5′-terminally labeled with ³²P were incubated with UvrABC proteins in the UvrABC buffer in the presence of 1-mM ATP at 37°C for the indicated time periods. The incision products were analyzed on a 12% urea PAGE under denaturing conditions. (A) AAF-50 bp; (B) Tg-51 bp. (C) Kinetics of UvrABC incisions based upon the data in (A) and (B).