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Binding of human XPC/HR23B and XPA proteins to the site-specific 5R-Tg paired with dA substrate. In (A) and (B), XPA (A) or XPC/HR23B (B) proteins at the indicated concentrations were incubated with 4-nM substrates at 30°C for 30 min in XPA binding buffer and then analyzed on 3.5% polyacrylamide native gels by gel mobility shift assays (XPA, 4°C; XPC/HR23B, room temperature). (C) and (D) show the binding curves generated from the data in (A) and (B).
