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. 2009 Oct 27;38(2):e7. doi: 10.1093/nar/gkp897

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© The Author(s) 2009. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Cloned sequence data following SPEX amplification from the Amerindian (PE40, PE187) and Iceman (HB50) aDNA extracts using the 16225-Down SPEX primer set (Supplementary Table S1), as for the amplification strategy described in Supplementary Figure S1c. Nucleotide positions of the hg K (np 16224) and hg B4b (np 16217) SNPs are each highlighted by a red asterisk above the reverse complement of the reference sequence (Ref). Diagnostic hg K (np 16224) and hg B4b (np 16217) sites in SPEX-generated sequence data are highlighted in yellow; C > U-derived G > A transitions are highlighted in green; any other base changes are highlighted in grey.