Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
(A) Biotinylated-GST titration curve was used to identify the ‘hook’ point of AlphaScreen assay. (B) Chromodomain proteins CDY2B, HP1β, and MPP8 demonstrated binding selectivity for biotinylated histone H3 peptides trimethylated at Lys9 over peptides trimethylated at Lys4. GST-tagged proteins (100 nM) were incubated with either 100 nM b-H3K4me3 (white) or b-H3K9me3 (black). Assay background was low, as no signal was observed in the absence of peptide (gray).
