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. 2009 Nov 6;38(2):e11. doi: 10.1093/nar/gkp899

Figure 2.

Figure 2.

AlphaScreen assay was miniaturized to a volume of 3 µl/well for high-throughput screening. Comparable binding of 20–200 nM b-H3K4me3 (squares) and b-H3K9me3 (circles) peptides to 100 nM GST-HP1β was observed in 384-well (A) and 1536-well (B) plate formats. Data in 1536-well plate format (B) are shown as the mean and standard deviation of four replicate measurements.