Figure 3.

Replication of linear substrates. (A) BsmBI linearized 3.5 kb NT pRST5_NT (5_NT) was incubated with egg cytosol at 1.5 ng/µl in the presence of either buffer control, 5 mM Caffeine, 100 ng/µl Geminin or Caffeine and Geminin for 30 min before addition of 2-fold volumes of NPE and (α-³²P)dCTP. Reactions were processed after 60 min for native agarose gel electrophoresis. The radioactive signal of three independent experiments were quantified by Phosphorimager (B). Values for untreated conditions are set to 1. P-values were calculated using a t-test.