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. 2009 Nov 11;38(2):477–487. doi: 10.1093/nar/gkp976

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© The Author(s) 2009. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — SMC1 depletion radiosensitizes HeLa cells. (A) Depletion of SMC1 in HeLa cells using RNA interference. Cells were transfected with two different siRNAs against SMC1 (SMC1-1 and SMC1-3), a non-targeting siRNA (siCo1) or untransfected. Protein levels were analysed by western blotting. The percentage of SMC1 levels with respect to the untransfected control is shown. (B) Immunofluorescence microscopic analysis of SMC1 depletion. Details as in (A). (C) Colony formation assay for control and SMC1-depleted cells. Cells were transfected with two different siRNAs against SMC1 (SMC1-1 and SMC1-3) and two different non-targeting siRNAs (siCo1 and siCo2). Error bars are standard errors of the mean from three experiments.