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. 2009 Nov 11;38(2):477–487. doi: 10.1093/nar/gkp976

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© The Author(s) 2009. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — Contribution of DNA damage kinases and/or the cohesin factors SMC1 and Rad21 to 53BP1 foci loss in G1 and late S/G2-phase cells. 1 Gy X-ray-induced 53BP1 foci were scored in G1- (CENP-F negative) and late S/G2-phase cells (CENP-F strongly positive) 4 h after 1 Gy X-irradiation. (A and B) Un-irradiated and irradiated G1-phase cells. (C and D) Un-irradiated and irradiated late S/G2-phase cells. Error bars are standard deviations from two experiments. (E) Depletion of Rad21 using RNA interference. Cells were transfected with siRNAs against Rad21 or non-targeting siRNA (lacZ). Protein levels were analysed by western blotting. Rad21-depleted cells contained less than 2% of the levels found in control cells.