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. Author manuscript; available in PMC: 2010 Dec 30.
Published in final edited form as: J Am Chem Soc. 2009 Dec 30;131(51):18467–18477. doi: 10.1021/ja907750r

Figure 2.

Figure 2

Two-step multi-enzyme chemoenzymatic synthesis of diasialyl oligosaccharides containing different sialic acid forms and various sialyl linkages. Enzymes: E. coli aldolase, Escherichia coli K12 sialic acid aldolase; NmCSS, Neisseria meningitidis CMP-sialic acid synthetase; PmST1, Pasteurella multocida sialyltransferase for the formation of α2–3-linked sialosides; Pd2,6ST, Photobacterium damsela α2–6-sialyltransferase for the formation of α2–6-linked sialosides; CstII, Campylobacter jejuni sialyltransferase for the formation of α2–8-linked sialosides. Compounds: Man, mannose; ManNAc, N-acetylmannosamine; CTP, cytidine 5′-triphosphate; Sia, sialic acid.