Figure 4.
Synthesis of disialyl GD3 oligosaccharides Neu5Acα2–8Siaα2–3GalβOR containing a terminal α2–8-linked Neu5Ac and different penultimate α2–3-linked sialic acid forms. Reagents and conditions: (i) Pyruvate, CTP, Mg2+, Tris-HCl buffer (pH 8.5), E. coli K12 sialic acid aldolase, NmCSS, and CstIIΔ32I53S. aDetermined by small-scale reaction using TLC analysis.