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. Author manuscript; available in PMC: 2010 Dec 30.
Published in final edited form as: J Am Chem Soc. 2009 Dec 30;131(51):18467–18477. doi: 10.1021/ja907750r

Figure 5.

Figure 5

Enzymatic preparation of GD3-type disialyl oligosaccharides Neu5Gc/KDNα2–8Neu5Gc/KDNα2–3LacβProN3 containing the combination of Neu5Gc and KDN. Reagents and conditions: (i) Pyruvate, CTP, Mg2+, Tris-HCl buffer (pH 8.5), E. coli K12 sialic acid aldolase, NmCSS, and PmST1; (ii) Pyruvate, CTP, Mg2+, Tris-HCl buffer (pH 8.5), E. coli K12 sialic acid aldolase, NmCSS, and CstIIΔ32I53S.