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. Author manuscript; available in PMC: 2010 Dec 18.
Published in final edited form as: ACS Chem Biol. 2009 Dec 18;4(12):1029–1038. doi: 10.1021/cb900210r

Fig. 2. The Sumo fusion-proteolysis strategy for obtaining membrane spanning N-Cys peptides.

Fig. 2

a) The peptide of interest is expressed as a sumo fusion. The fusion protein is cleaved by the sumo protease to generate the desired N-Cys peptide that is purified by RP-HPLC. b) SDS-PAGE of the sumo fusion protein (lane 1) and the proteolysis reaction (lane 2) with P, sumo protease; S, sumo protein; C, N-Cys KcsA (82–160) C-peptide. c) ES-MS of the purified N-Cys KcsA peptide (82–160). Inset, reconstructed spectrum (Expected mass = 10220.6).