Figure 2.

Primer Design Flowchart. A flowchart visualizing the main steps in our program. Starting with one sequence ('global mode') or several sequences ('targeted mode'), primers are enumerated and pairs are created. Thereafter, the most-homogeneous tuple set from the lists of tuples is determined containing exactly one tuple from each list. In case of empty candidate primer lists, candidate tuple lists or tuple sets, refinements are done if possible. Finally, results are returned by mail.