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. 2010 Jan 26;8(1):e1000288. doi: 10.1371/journal.pbio.1000288

Figure 7. Recombinant TAP38 directly dephosphorylates pLHCII in vitro.

Figure 7

(A) Equal amounts of pLHCII isolated from tap38-1 mutant plants, treated with or without recombinant TAP38, were separated by SDS-PAGE and immunodecorated with phosphothreonine-specific antibodies. NaF (10 mM) was added to specifically inhibit phosphatase activity. (B) A replicate gel of the samples as in (A) was stained with Coomassie Blue as a loading control. The recombinant TAP38 protein and LHCII bands are shown. (C) Densitometric quantification of the bands in (A), representing the phosphorylation levels of LHCII under the different conditions.