Table 1.

Mouse NTD mutants tested for responsiveness to Folate

Folate Responsive NTD Mutant Mice
Gene	Folate dose/route	Reference
Cart1 null	3 mg/kg i.p., 0.5–9.5 dpc	(Zhao and others, 1996)
Cited2 null	3 mg/kg i.p. 0–14 dpc	(Barbera and others, 2002)
Cd; Lrp6 gain of function	10 mg/kg chow Diet	(Carter and others, 2005)
Folr1 null	25 mg/kg/d gavage	(Piedrahita and others, 1999)
RFC1 reduced folate carrier null	50 mg/kg sc./d 0–18 dpc	(Gelineau-van Waes and others, 2008)
Sp2H; Pax3 Loss of function	200 ug/ml in culture	(Fleming and Copp, 1998)
Sp; Pax3	200 mg/kg chow Diet	(Wlodarczyk and others, 2006)

Folate Unresponsive NTD Mutant Mice
Gene	Folate dose/route	Reference
Axd mutant	33 mg/kg i.p, 8–10 dpc	(Essien and Wannberg, 1993)
Fkbp8 null	40 mg/kg Gavage 0–18.5 dpc	(Wong and others, 2008)
ct; Grhl3 loss of function	60 mg/kg folinic a. i.p. @ 9 dpc	(Seller, 1994)
Grhl3 null	as per Seller, 1994	(Ting and others, 2003)
SELH/Bc strain	10 mg/kg chow Diet	(Harris and Juriloff, 2005)
Efna5 null	Data not shown	(Holmberg and others, 2000)
Map3k4 null	25 mg/kg i.p./d, 6.5–9.5 dpc	(Chi and others, 2005)
Nog	10 mg/kg i.p./d, 7.5–9.5 dpc	(Stottmann and others, 2006)
Ski null	10 mg/kg chow Diet	(Ernest and others, 2006)

i.p., intraperitoneal; sc., subcutaneous; d, day; dpc, days postcoitum